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After processing, the water in the tissue is replaced with wax. The sections are kept in hot wax until they are flattened and cooled in a thick block of wax to hold them for cutting.
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Bio-Photometrics Tissuescope 4000 Brightfield and reflection confocal microscope. Will do full colour and fluorescence imaging of up to 5"x7" slides. Seen here aer 3- 2"x3" slides being imaged. Next week- we will convert a slide image to gigapanformat.
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GE DS Digital mammography unit with magnification stand Each slice is imaged to demonstrate that microcalcifications seen on original mammogram have been removed in lumpectomy.
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Telecentric lens and camera used to image each slice before processing. This is used forr image registration. 3 sizes of lenses are available depending on sample size. The lens gives accurate distance measurement independent of tissue thickness (about 5 cm depth of field).
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Tissue sliced (breadloafed) at 3 mm serial sections Colours indicate orientation of tissue in patient during surgery
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This lab is used for tissue preparation of whole-mount slides. Tissue is sliced at 3-5 mm thickness and then fixed and embedded. Breast slides of 5" x 7" are serial sectioned to generate 3-D volumes of tissue. They can be H&E stained or immuno-stained. Left side- Stainless counter for handling fresh ti...
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Left side- Automatic slide stainer- H&E 2 Leica SM2500 sledge macrotomes for whole mount sildes (5" x 7" breast) Leica SM2600 miller and macrotome attachment for resin mount Standard microtome for 1 x 3 and 2 x 3 paraffin sections
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