1. Skip to navigation
  2. Skip to content
  3. Skip to sidebar

whereRU: Aresty Poster 22 - Neuroprotective Effects of Cypin on Spinal Cord Injuries by Aresty Posters 2009

Want to add this gigapan to your favorites? Log In or Sign Up now.

Log In now to add this Gigapan to a group gallery.

About This Gigapan

Taken by
Aresty Posters 2009 Aresty Posters 2009
Explore score
0.06 Gigapixels
Date added
Jun 05, 2009
Date taken
Jun 04, 2009

Neuroprotective Effects of Cypin on Spinal Cord Injuries

During spinal cord injury (SCI), there is an extremely high release of the neurotransmitter glutamate. This leads to activation of glutamate NMDA (N-methyl-D-aspartate) receptors and to the production of reactive oxygen species (ROS) that have detrimental effects on the neurons, often resulting in neuronal death. Interestingly, interfering with the interactions between NMDA receptor and PSD-95 (postsynaptic density-95), a synaptic structural protein that enhances NMDA receptor surface expression and signaling efficiency, may have neuroprotective effects. Cypin (cytosolic protein interacting with PSD-95) is a major binding partner of PSD-95. PSD-95 belongs to a family of proteins called MAGUK (membrane-associate guanylate kinase), involved with the recruitment of signaling molecules to the synapse. Therefore, PSD-95 and cypin play crucial roles in the clustering of molecules that respond to neurotransmitters at the synaptic site. Thus, with previous data from the Firestein laboratory showing that overexpression of cypin may indirectly downregulate the signaling strength of NMDA receptors, we sought to investigate the role of cypin in limiting damaging effects of SCI. For this purpose, we analyzed the effect of cypin overexpression on PSD-95 distribution in spinal cord neurons and the neuroprotective role of cypin overexpression in these neurons after glutamate-induced toxicity. Here we show that overexpression of cypin does decrease the number of PSD-95 clusters in cultured spinal cord neurons. However, in our experimental conditions, we did not find that the overexpression of cypin significantly improves neuronal survival.

Sean Lo
Dr. Bonnie Firestein Lab

Gigapan Comments (0)

Toggle Minimize gigapan_comment
The GigaPan EPIC Series, Purchase an GigaPan EPIC model and receive GigaPan Stitch complimentary

Where in the World is this GigaPan?

Sorry, this gigapan has no location information.

Member Log In