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About This GigaPan
Toggle- Taken by
-
Gord Mawdsley
- Explore score
- 48
- Size
- 0.14 Gigapixels
- Views
- 3327
- Date added
- February 11, 2009
- Date taken
- February 10, 2009
- Categories
- Galleries
- Laboratories
- Competitions
- Tags
- laboratory, room, pathology, tissue, 3d
- Description
-
This lab is used for tissue preparation of whole-mount slides.
Tissue is sliced at 3-5 mm thickness and then fixed and embedded.
Breast slides of 5" x 7" are serial sectioned to generate 3-D volumes of tissue. They can be H&E stained or immuno-stained.
Left side- Stainless counter for handling fresh tissue - Slicer in fume hood
centre - Tissue processors, standard and microwave.
Right side - embedding and staining section.Standard microtome for 1 x 3 and 2 x 3 paraffin sections
Stitcher Notes
ToggleMinimizeGigaPan Stitcher version 0.4.3864 (Windows)
Panorama size: 138 megapixels (28614 x 4832 pixels)
Input images: 39 (13 columns by 3 rows)
Field of view: 298.6 degrees wide by 50.4 degrees high (top=18.4, bottom=-32.0)
Settings:
All default settings
Original image properties:
Camera make: Canon
Camera model: Canon PowerShot SD1100 IS
Image size: 3264x2448 (8.0 megapixels)
Capture time: 2009-02-10 11:11:03 - 2009-02-10 11:15:50
Aperture: f/3.5
Exposure time: 0.04 - 0.3
ISO: 100
Focal length (35mm equiv.): 58.6 mm
Digital zoom: off
White balance: Fixed
Exposure mode: Automatic
Horizontal overlap: 33.8 to 39.2 percent
Vertical overlap: 48.5 to 51.6 percent
Computer stats: 2046.42 MB RAM, 1 CPUs
Total time 1:40:57 (2:35 per picture)
Alignment: 3:44, Projection: 8:02, Blending: 1:29:09
Stoney Vintson (February 11, 2009, 03:41PM )
This is great. I always wanted to know more about how they prepared histological samples. I have seen series of brain tissue from a microtome that allows you to understand the tissue much better than a single slice. Thanks for posting this :) Would you be able to make some snapshots showing where the microtome and other steps.